9EU3

GH29A alpha-L-fucosidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-04
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.8731
Spacegroup name	P 32 2 1
Unit cell lengths	146.871, 146.871, 196.703
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	58.280 - 2.280
R-factor	0.2137
Rwork	0.212
R-free	0.24310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.642
Data reduction software	autoPROC
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.280	2.360
High resolution limit [Å]	2.280	2.280
Rmerge	0.226	1.000
Rmeas	0.232	1.000
Rpim	0.051	1.000
Number of reflections	111956	11067
<I/σ(I)>	7.92	0.28
Completeness [%]	97.7	78.11
Redundancy	20.7	21.3
CC(1/2)	0.999	0.369

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	293	8% Ethylene glycol 0.1M HEPES pH 7.3 10% PEG 8000