9EU2
GH29A alpha-L-fucosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-04 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.8731 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 147.912, 147.912, 197.086 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.020 - 1.840 |
| R-factor | 0.1625 |
| Rwork | 0.162 |
| R-free | 0.18800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.952 |
| Data reduction software | autoPROC |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.020 | 1.906 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.132 | 1.000 |
| Rmeas | 0.135 | 1.000 |
| Rpim | 0.030 | 0.503 |
| Number of reflections | 215060 | 21308 |
| <I/σ(I)> | 18 | 1.47 |
| Completeness [%] | 99.9 | 99.98 |
| Redundancy | 20.7 | 20.7 |
| CC(1/2) | 0.999 | 0.717 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 293 | 20% Ethylene glycol 0.1M HEPES pH 7.3 10% PEG 8000 |
