9EU1
GH29A alpha-L-fucosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-04 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.8731 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 139.167, 139.167, 110.233 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.670 - 1.920 |
| R-factor | 0.1839 |
| Rwork | 0.183 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.025 |
| Data reduction software | autoPROC |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.670 | 1.989 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.113 | 1.000 |
| Rmeas | 0.116 | 1.000 |
| Rpim | 0.025 | 0.500 |
| Number of reflections | 93816 | 9287 |
| <I/σ(I)> | 17.43 | 1.46 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 20.8 | 21 |
| CC(1/2) | 0.999 | 0.617 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2M Ammonium sulphate 0.1M Bis-tris pH 7.5 2.2M Ammonium sulphate |
