9ETL
Mouse CNPase catalytic domain with nanobody 8D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.300, 46.541, 91.976 |
| Unit cell angles | 95.66, 100.11, 90.08 |
Refinement procedure
| Resolution | 45.050 - 1.500 |
| R-factor | 0.1796 |
| Rwork | 0.178 |
| R-free | 0.22930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.006 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21rc1_5127) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.590 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmeas | 0.091 | 2.368 |
| Number of reflections | 190179 | 13946 |
| <I/σ(I)> | 6.9 | 0.5 |
| Completeness [%] | 89.7 | 89.4 |
| Redundancy | 2.7 | |
| CC(1/2) | 0.997 | 0.224 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M phosphate-citrate pH 4.2, 18% PEG8000, 0.35M NaCl |
