9ETK
Crystal structure of Vibrio cholerae RNase AM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-01 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.03285 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 47.464, 163.919, 39.607 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.590 - 1.480 |
| R-factor | 0.1518 |
| Rwork | 0.151 |
| R-free | 0.18000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.886 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.590 | 1.500 |
| High resolution limit [Å] | 1.479 | 1.480 |
| Number of reflections | 99127 | 65 |
| <I/σ(I)> | 12.45 | |
| Completeness [%] | 99.3 | 91.43 |
| Redundancy | 6.8 | 4.4 |
| CC(1/2) | 0.999 | 0.031 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 292 | 0.1 M Bis-Tris (pH 5.0), 0.2 M ammonium sulfate and 22.5 % PEG 3350 |
