Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.89842 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.832, 133.200, 147.089 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 98.928 - 2.641 |
| Rwork | 0.220 |
| R-free | 0.22160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.625 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 133.200 | 133.200 | 2.720 |
| High resolution limit [Å] | 2.620 | 9.800 | 2.620 |
| Rmerge | 0.300 | 0.066 | 2.803 |
| Rmeas | 0.323 | 0.071 | 3.011 |
| Rpim | 0.120 | 0.026 | 1.098 |
| Number of reflections | 43095 | 981 | 4611 |
| <I/σ(I)> | 6.2 | 21.5 | 0.7 |
| Completeness [%] | 96.9 | ||
| Redundancy | 13.6 | 12.3 | 14.4 |
| CC(1/2) | 0.994 | 0.999 | 0.718 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | Protein at 10 mg/ml. 0.6 to 0.8 M KCl, 0.9 to 1.2 M (NH4)2SO4, and 100 mM HEPES pH 7.0 |
