9ET3
CDK2-cyclin A in complex with FragLite 10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-05-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91839 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.121, 133.849, 148.042 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.483 - 2.340 |
| Rwork | 0.183 |
| R-free | 0.22140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.685 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 148.040 | 2.400 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.116 | 1.624 |
| Number of reflections | 59478 | 4870 |
| <I/σ(I)> | 16.8 | 1.9 |
| Completeness [%] | 94.4 | 100 |
| Redundancy | 13.5 | 14.1 |
| CC(1/2) | 0.999 | 0.658 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | Protein at 10 mg/ml. 0.6 to 0.8 M KCl, 0.9 to 1.2 M (NH4)2SO4, and 100 mM HEPES pH 7.0 |
