9ERW

Mouse CNPase catalytic domain with nanobody 8C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033
Spacegroup name	P 1 21 1
Unit cell lengths	39.860, 54.170, 80.400
Unit cell angles	90.00, 96.36, 90.00

Refinement procedure

Resolution	44.840 - 1.730
R-factor	0.192
Rwork	0.191
R-free	0.21520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.050
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_5127)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.770
High resolution limit [Å]	1.730	1.730
Rmeas	0.080	2.256
Number of reflections	35411	2582
<I/σ(I)>	11.8	1
Completeness [%]	99.1	98.7
Redundancy	5.6
CC(1/2)	0.998	0.359

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	0.1 M MIB pH 8.0, 25% PEG1500