9ERH

Uhgb_MS mannoside synthase from an unknown human gut bacterium in complex with 1,2-beta-mannobiose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97926
Spacegroup name	P 61
Unit cell lengths	178.183, 178.183, 83.675
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.650
Rwork	0.169
R-free	0.18160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.732
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	154.310	2.790
High resolution limit [Å]	2.650	2.650
Rmerge	0.076	0.752
Number of reflections	44200	6415
<I/σ(I)>	7.1
Completeness [%]	100.0
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	285