9EQQ
Uhgb_MS mannoside synthase from an unknown human gut bacterium in complex with Mannose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.978565 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 176.629, 176.629, 83.539 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.586 - 2.550 |
| Rwork | 0.179 |
| R-free | 0.20670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.392 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.586 | 2.690 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.102 | 0.718 |
| Number of reflections | 1030781 | 3653 |
| <I/σ(I)> | 4.8 | |
| Completeness [%] | 100.0 | |
| Redundancy | 40.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 285 | 0.2 M Calcium acetate, 0.1 M Imidazole pH 8.0, 20 %PEG 1000 |
