9EQH
WWP2 WW2-2,3-linker-HECT (WWP2-LH)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.009, 90.270, 111.177 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.079 - 2.050 |
| Rwork | 0.213 |
| R-free | 0.27490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.222 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.079 | 5.552 |
| High resolution limit [Å] | 2.046 | 2.046 |
| Rmerge | 0.190 | |
| Rmeas | 0.205 | |
| Rpim | 0.075 | |
| Number of reflections | 28618 | 1419 |
| <I/σ(I)> | 6.9 | |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 6.7 | |
| CC(1/2) | 0.994 | 0.486 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 100 mM MMT pH 6.0, 25 % PEG1500 |
