9EQA
Iron loaded mitochondrial ferritin exposed to oxygen for 20 minutes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-07 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.975 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 182.526, 182.526, 182.526 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 105.380 - 1.970 |
| R-factor | 0.1903 |
| Rwork | 0.188 |
| R-free | 0.23380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.240 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 105.380 | 2.040 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.151 | |
| Rmeas | 0.154 | |
| Rpim | 0.026 | 0.681 |
| Number of reflections | 32993 | 1862 |
| <I/σ(I)> | 11.82 | 0.3 |
| Completeness [%] | 94.8 | 59 |
| Redundancy | 20 | 12.9 |
| CC(1/2) | 0.999 | 0.462 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 289 | 2 M magnesium chloride 100 mM sodium chloride 3 mM sodium azide 100 mM bicine 60 mM ferrous chloride pH 9.0 |
