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9EQ9

Iron loaded mitochondrial ferritin, anaerobic

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsDIAMOND BEAMLINE I03
Synchrotron siteDiamond
BeamlineI03
Temperature [K]100
Detector technologyPIXEL
Collection date2020-06-07
DetectorDECTRIS EIGER2 XE 16M
Wavelength(s)0.975
Spacegroup nameF 4 3 2
Unit cell lengths177.430, 177.430, 177.430
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution51.220 - 1.840
R-factor0.2064
Rwork0.204
R-free0.24740
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.013
RMSD bond angle1.245
Data reduction softwarexia2
Data scaling softwareAimless
Phasing softwarePHENIX
Refinement softwarePHENIX (1.20.1_4487)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]51.2201.906
High resolution limit [Å]1.8401.841
Number of reflections213312088
<I/σ(I)>0.64
Completeness [%]89.8
Redundancy21.3
CC(1/2)0.9900.364
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP92890.1 M bicine 2 M magnesium chloride 100 mM sodium chloride 60 mM ferrous chloride 3 mM sodium azide pH 9.0

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