9EQ9

Iron loaded mitochondrial ferritin, anaerobic

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-07
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.975
Spacegroup name	F 4 3 2
Unit cell lengths	177.430, 177.430, 177.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.220 - 1.840
R-factor	0.2064
Rwork	0.204
R-free	0.24740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.245
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.220	1.906
High resolution limit [Å]	1.840	1.841
Number of reflections	21331	2088
<I/σ(I)>	0.64
Completeness [%]	89.8
Redundancy	21.3
CC(1/2)	0.990	0.364

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	289	0.1 M bicine 2 M magnesium chloride 100 mM sodium chloride 60 mM ferrous chloride 3 mM sodium azide pH 9.0