9EQ9
Iron loaded mitochondrial ferritin, anaerobic
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-07 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.975 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 177.430, 177.430, 177.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.220 - 1.840 |
R-factor | 0.2064 |
Rwork | 0.204 |
R-free | 0.24740 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.245 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.220 | 1.906 |
High resolution limit [Å] | 1.840 | 1.841 |
Number of reflections | 21331 | 2088 |
<I/σ(I)> | 0.64 | |
Completeness [%] | 89.8 | |
Redundancy | 21.3 | |
CC(1/2) | 0.990 | 0.364 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 289 | 0.1 M bicine 2 M magnesium chloride 100 mM sodium chloride 60 mM ferrous chloride 3 mM sodium azide pH 9.0 |