9EQ6

Cachd1 and FZD5 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0056
Spacegroup name	P 41 21 2
Unit cell lengths	123.107, 123.107, 367.629
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	91.910 - 2.720
R-factor	0.2597
Rwork	0.258
R-free	0.28630
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.515
Data reduction software	xia2.multiplex
Data scaling software	xia2.multiplex
Phasing software	AutoSol
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	123.110	2.770
High resolution limit [Å]	2.720	2.720
Number of reflections	77011	3740
<I/σ(I)>	17
Completeness [%]	100.0
Redundancy	46.9
CC(1/2)	0.999	0.499

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	296	1.0 M Ammonium sulphate; 0.1 M MES pH6.5