9EP3

Structure of the SA2/SCC1/WAPL complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-01
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9655
Spacegroup name	P 21 21 21
Unit cell lengths	78.433, 107.440, 181.913
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.970 - 3.080
R-factor	0.2265
Rwork	0.224
R-free	0.26820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.522
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.970	3.260
High resolution limit [Å]	3.080	3.080
Number of reflections	28667	2797
<I/σ(I)>	9.2
Completeness [%]	98.6	97.4
Redundancy	4.4
CC(1/2)	0.990	0.375

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.06M Morpheus Divalents mix, 0.1M Morpheus buffer system 1, 48% (v/v) Morpheus EOD_P8K