9EO5
X-ray structure of the adduct formed upon reaction of picoplatin with bovine pancreatic ribonuclease (structure C)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.750, 32.690, 73.540 |
| Unit cell angles | 90.00, 90.46, 90.00 |
Refinement procedure
| Resolution | 50.424 - 1.990 |
| Rwork | 0.191 |
| R-free | 0.24260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.536 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.424 | 2.080 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.161 | 0.161 |
| Number of reflections | 16745 | 844 |
| <I/σ(I)> | 7.3 | 2.1 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 5.9 | 5.8 |
| CC(1/2) | 0.988 | 0.638 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 22% PEG4000, 0.1 M sodium citrate pH 5.1 |
