9ENJ
L-amino acid oxidase 4 (HcLAAO4) from the fungus Hebeloma cylindrosporum in complex with L-glutamate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-22 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.96770 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.760, 130.790, 107.300 |
Unit cell angles | 90.00, 95.66, 90.00 |
Refinement procedure
Resolution | 40.620 - 2.100 |
R-factor | 0.1751 |
Rwork | 0.174 |
R-free | 0.19500 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.002 |
RMSD bond angle | 0.520 |
Data reduction software | XDS (VERSION Jun 30, 2023 BUILT=20230630) |
Data scaling software | XSCALE (VERSION Jun 30, 2023 BUILT=20230630) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmeas | 0.106 | 1.901 |
Number of reflections | 134327 | 9924 |
<I/σ(I)> | 12.45 | 1.27 |
Completeness [%] | 99.2 | 99.9 |
Redundancy | 7.1 | 7.36 |
CC(1/2) | 0.997 | 0.500 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | protein: 10 mg/ml; reservoir solution: 2.05 M (NH4)2SO4, 0.1 M sodium citrate, pH 7.0; drop with 1000 nl protein + 500 nl reservoir |