9ENH
L-amino acid oxidase 4 (HcLAAO4) from the fungus Hebeloma cylindrosporum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-01-23 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.88560 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 156.636, 158.283, 217.592 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.560 - 2.300 |
R-factor | 0.213 |
Rwork | 0.211 |
R-free | 0.24580 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.473 |
Data reduction software | XDS (VERSION Jun 30, 2023 BUILT=20230630) |
Data scaling software | STARANISO (2.4.9 (25-Oct-2023)) |
Phasing software | PHASER |
Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.600 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.109 | 0.934 |
Rmeas | 0.114 | 1.039 |
Rpim | 0.033 | 0.432 |
Number of reflections | 84158 | 4209 |
<I/σ(I)> | 13.2 | 1.8 |
Completeness [%] | 70.5 | 21.8 |
Redundancy | 11.2 | 5.8 |
CC(1/2) | 0.997 | 0.410 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | protein: 10 mg/ml; reservoir solution: 2.25 M (NH4)2SO4, 0.1 M sodium citrate, pH 6.5; drop with 200 nl protein + 100 nl reservoir |