9ENH

L-amino acid oxidase 4 (HcLAAO4) from the fungus Hebeloma cylindrosporum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.88560
Spacegroup name	C 2 2 21
Unit cell lengths	156.636, 158.283, 217.592
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.560 - 2.300
R-factor	0.213
Rwork	0.211
R-free	0.24580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.473
Data reduction software	XDS (VERSION Jun 30, 2023 BUILT=20230630)
Data scaling software	STARANISO (2.4.9 (25-Oct-2023))
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.600	2.440
High resolution limit [Å]	2.300	2.300
Rmerge	0.109	0.934
Rmeas	0.114	1.039
Rpim	0.033	0.432
Number of reflections	84158	4209
<I/σ(I)>	13.2	1.8
Completeness [%]	70.5	21.8
Redundancy	11.2	5.8
CC(1/2)	0.997	0.410

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	protein: 10 mg/ml; reservoir solution: 2.25 M (NH4)2SO4, 0.1 M sodium citrate, pH 6.5; drop with 200 nl protein + 100 nl reservoir