9EN1

FliG interacting domain of SpFlhF (FID)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976990
Spacegroup name	P 43
Unit cell lengths	106.530, 106.530, 106.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.680 - 3.010
R-factor	0.2365
Rwork	0.235
R-free	0.26840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.582
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.680	37.680	3.080
High resolution limit [Å]	3.010	13.440	3.010
Rmerge	0.077	0.042	2.778
Rmeas	0.080	0.044	2.884
Number of reflections	23814	280	1720
<I/σ(I)>	18.63
Completeness [%]	99.9
Redundancy	13.5
CC(1/2)	0.999	0.998	0.408

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	1.6M Sodium citrate pH 6.5