9EM0
OPR3 wild type in its dimeric form with special L6 conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-08 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.8731 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.695, 89.659, 80.148 |
| Unit cell angles | 90.00, 104.17, 90.00 |
Refinement procedure
| Resolution | 47.460 - 2.200 |
| R-factor | 0.2496 |
| Rwork | 0.249 |
| R-free | 0.27680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.460 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.460 | 2.090 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rpim | 0.230 | 1.710 |
| Number of reflections | 102435 | 10325 |
| <I/σ(I)> | 2.47 | |
| Completeness [%] | 97.2 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.960 | 0.150 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MES/Tris (6.5), 50 mM ammonium sulfate, 16% PEG8000 |
