9ELZ

CRYSTAL STRUCTURE OF RHESUS MACAQUE (MACACA MULATTA) IGG2 FC FRAGMENT- FC-GAMMA RECEPTOR IIA COMPLEX H131 VARIANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95369
Spacegroup name	P 65 2 2
Unit cell lengths	128.123, 128.123, 253.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.920 - 3.200
R-factor	0.277
Rwork	0.275
R-free	0.30600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.334
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.200	3.200
Rmerge	0.212
Rpim	0.048	0.481
Number of reflections	21259	807
<I/σ(I)>	17.9	0.7
Completeness [%]	98.2	77
Redundancy	17.8	5.4
CC(1/2)	0.990	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	12% PEG 8000, 0.1 M magnesium acetate, 0.1 M MOPS pH 7.5