9ELW

CRYSTAL STRUCTURE OF RHESUS MACAQUE (MACACA MULATTA) IGG1 FC FRAGMENT- FC-GAMMA RECEPTOR IIA COMPLEX H131 VARIANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-18
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97946
Spacegroup name	P 65 2 2
Unit cell lengths	127.164, 127.164, 254.227
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.170 - 3.550
R-factor	0.295
Rwork	0.293
R-free	0.33700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.751
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.610
High resolution limit [Å]	3.550	3.550
Rmerge	0.348
Rpim	0.086	0.918
Number of reflections	15333	744
<I/σ(I)>	28.5	0.9
Completeness [%]	99.9	99.2
Redundancy	17.1
CC(1/2)	0.980	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	15% PEG 4000, 0.1 M HEPES pH 7.0