9ELU
CRYSTAL STRUCTURE OF RHESUS MACAQUE (MACACA MULATTA) IGG1 FC FRAGMENT- FC-GAMMA RECEPTOR IIA COMPLEX P131 VARIANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-10 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 127.528, 127.528, 256.137 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.370 - 2.650 |
| R-factor | 0.28 |
| Rwork | 0.277 |
| R-free | 0.32700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.449 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.123 | |
| Rpim | 0.029 | 0.647 |
| Number of reflections | 36316 | 1723 |
| <I/σ(I)> | 57.5 | 0.9 |
| Completeness [%] | 99.9 | 98.2 |
| Redundancy | 18.5 | 12 |
| CC(1/2) | 1.000 | 0.360 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 10% PEG 5000 MME, 12% isopropanol, 0.1M MES pH 6.5 |
