9EL6
Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation F90L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.249, 74.326, 130.651 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.580 - 2.250 |
| R-factor | 0.2455 |
| Rwork | 0.239 |
| R-free | 0.30150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.860 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3247) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.580 | 2.328 |
| High resolution limit [Å] | 2.248 | 2.248 |
| Rpim | 0.067 | 0.512 |
| Number of reflections | 20150 | 1915 |
| <I/σ(I)> | 6 | |
| Completeness [%] | 99.0 | |
| Redundancy | 5.7 | |
| CC(1/2) | 1.000 | 0.725 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1M PCTP (pH8.0), 25% (w/v) PEG 1500 |
