9EJY
Human hnRNPR extended eRRM1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.72929 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 77.023, 77.023, 52.893 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.130 - 1.900 |
| R-factor | 0.197 |
| Rwork | 0.194 |
| R-free | 0.22180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.953 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX (1.21.2-5419) |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.130 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.033 | 2.174 |
| Rmeas | 0.035 | |
| Rpim | 0.011 | 0.686 |
| Number of reflections | 14604 | 1445 |
| <I/σ(I)> | 40.7 | 1.5 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 20.9 | 21 |
| CC(1/2) | 1.000 | 0.626 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.04 M potassium phosphate, monobasic; 20% v/v glycerol; 8% w/v PEG 8,000; 1:1 drop ratio; 16 mg/mL protein |
