9EFP

Crystal Structure of Saccharomyces cerevisiae Sec14p in complex with phosphatidylcholine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9202
Spacegroup name	P 32 2 1
Unit cell lengths	74.400, 74.400, 195.860
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.860 - 1.830
R-factor	0.1859
Rwork	0.172
R-free	0.20840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.024
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.860	1.893
High resolution limit [Å]	1.830	1.830
Rmeas	0.143	0.568
Number of reflections	56267	5567
<I/σ(I)>	5.92	1.83
Completeness [%]	99.7	99.96
Redundancy	5.2
CC(1/2)	0.985	0.606

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	289.15	0.1 M Bis-Tris, 30-40% v/v PEG 400, pH 5.5-pH 6.5