9EFF
Crystal Structure of a nucleoid-associated protein (UBP) bound to DNA from Sulfolobus islandicus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2022-03-03 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 0.91165 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.742, 136.051, 38.380 |
| Unit cell angles | 90.00, 109.12, 90.00 |
Refinement procedure
| Resolution | 36.260 - 1.820 |
| R-factor | 0.2014 |
| Rwork | 0.200 |
| R-free | 0.22820 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.159 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.030 | 1.860 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmeas | 0.115 | 1.040 |
| Rpim | 0.043 | 0.416 |
| Number of reflections | 31882 | 1535 |
| <I/σ(I)> | 11.8 | 1.7 |
| Completeness [%] | 98.4 | 96.4 |
| Redundancy | 7 | 6.1 |
| CC(1/2) | 0.995 | 0.884 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | Tris-HCl 0.05 M pH 8.0 - 8.5, Potassium Chloride 0.1 M, Magnesium Chloride 0.01 M and PEG400 26 - 30% |
