9EFE
Crystal Structure in space group P21 of a nucleoid-associated protein (UBP) from Sulfolobus islandicus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2020-09-24 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1.24984 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.896, 165.538, 47.638 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.640 - 2.590 |
| R-factor | 0.2257 |
| Rwork | 0.222 |
| R-free | 0.25530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.696 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.640 | 2.600 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmeas | 0.086 | 1.216 |
| Rpim | 0.045 | 0.636 |
| Number of reflections | 41929 | 417 |
| <I/σ(I)> | 13.2 | 1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.7 | 3.6 |
| CC(1/2) | 0.999 | 0.447 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | NaAc 0.1 M pH 4.8 - 5.2, LiCl 0.2 M or MgCl2 0.2 M, and PEG6000 18 - 24% |
