9EFD
Crystal Structure in space group C2221 of a nucleoid-associated protein (UBP) from Sulfolobus islandicus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2020-09-24 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.24984 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 95.010, 159.670, 47.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.430 - 2.740 |
R-factor | 0.2679 |
Rwork | 0.268 |
R-free | 0.27790 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.462 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.310 | 2.860 |
High resolution limit [Å] | 2.730 | 2.730 |
Rmeas | 0.119 | 1.986 |
Rpim | 0.032 | 0.557 |
Number of reflections | 9913 | 1279 |
<I/σ(I)> | 22.6 | 1.9 |
Completeness [%] | 99.6 | 99.5 |
Redundancy | 13.8 | 13.2 |
CC(1/2) | 0.999 | 0.894 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.2 M LiCl NaAc 0.1 M pH=5 PEG6000 18-24% |