9EE7

Folded domains of Xrs2 from S.cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979330
Spacegroup name	P 61 2 2
Unit cell lengths	84.843, 84.843, 316.716
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	71.580 - 2.380
R-factor	0.2987
Rwork	0.298
R-free	0.31150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.120
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.580	2.434
High resolution limit [Å]	2.380	2.380
Rmerge	0.141	3.608
Rmeas		3.701
Rpim	0.031	0.820
Number of reflections	27755	1350
<I/σ(I)>	16.4	1.1
Completeness [%]	100.0
Redundancy	21.7
CC(1/2)	0.999	0.444

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	3.0 M NaCl, 0.1M Tris, pH 8.3