9EDQ

GX/NPIH26 bat norovirus protruding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-19
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.95372
Spacegroup name	P 32 2 1
Unit cell lengths	67.490, 67.490, 238.760
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.110 - 1.630
R-factor	0.1639
Rwork	0.162
R-free	0.19380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.796
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.750	1.660
High resolution limit [Å]	1.630	1.630
Rmerge	0.145	1.654
Rmeas	0.151	1.721
Rpim	0.041	0.467
Total number of observations	1071388	49582
Number of reflections	79732	3791
<I/σ(I)>	11.1	1.3
Completeness [%]	99.9
Redundancy	13.4	13.1
CC(1/2)	0.999	0.639

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	1.6 M magnesium sulfate heptahydrate, 0.1 M MES (pH 6.5)