9EDN
GII.23: Loreto1847 norovirus protruding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.140, 80.960, 71.790 |
| Unit cell angles | 90.00, 113.44, 90.00 |
Refinement procedure
| Resolution | 40.480 - 1.220 |
| R-factor | 0.1238 |
| Rwork | 0.123 |
| R-free | 0.14630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.8) |
| Phasing software | PHASER (9.0.002) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.590 | 1.230 |
| High resolution limit [Å] | 1.210 | 1.210 |
| Rmerge | 0.121 | 0.695 |
| Rmeas | 0.141 | 0.803 |
| Rpim | 0.071 | 0.396 |
| Total number of observations | 19626 | |
| Number of reflections | 185707 | 5677 |
| <I/σ(I)> | 7.8 | 2.1 |
| Completeness [%] | 97.3 | |
| Redundancy | 3.7 | 3.5 |
| CC(1/2) | 0.991 | 0.688 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1 M sodium acetate (pH 4.6), 8% w/v PEG 4000 |
