9EDK

Streptavidin-S112C-T114V bound to Cu(II)dpea cofactor

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	I 41 2 2
Unit cell lengths	57.760, 57.760, 183.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.990 - 1.450
R-factor	0.1816
Rwork	0.181
R-free	0.18960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.569
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.990	1.470
High resolution limit [Å]	1.450	1.450
Rmerge	0.059	0.643
Rmeas		0.755
Rpim	0.034	0.385
Number of reflections	27339	1329
<I/σ(I)>	10.4	1.6
Completeness [%]	97.2	96.8
Redundancy	3.5
CC(1/2)	0.998	0.656

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	298	ammonium sulfate, sodium acetate