9EDI

Streptavidin-S112C-L124A bound to Cu(II)dpea cofactor

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97648
Spacegroup name	I 41 2 2
Unit cell lengths	57.834, 57.834, 184.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.090 - 1.500
R-factor	0.1734
Rwork	0.172
R-free	0.20210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.190
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.090	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.078	1.105
Rmeas	0.080	1.136
Rpim	0.018	0.262
Number of reflections	25671	1262
<I/σ(I)>	23.3
Completeness [%]	100.0
Redundancy	18.6
CC(1/2)	1.000	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	ammonium sulfate, sodium acetate