9EDH

Streptavidin-S112C-T114F bound to Cu(II)dpea cofactor

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97648
Spacegroup name	I 41 2 2
Unit cell lengths	57.720, 57.720, 183.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.900 - 1.720
R-factor	0.1897
Rwork	0.188
R-free	0.22160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.196
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.900	1.750
High resolution limit [Å]	1.720	1.720
Rmerge	0.106	0.693
Rpim	0.054	0.470
Number of reflections	16927	893
<I/σ(I)>	8.5	1.9
Completeness [%]	99.5	100
Redundancy	4.7
CC(1/2)	0.987	0.769

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	298	ammonium sulfate, sodium acetate