9EDG

Streptavidin-WT bound to Cu(II)dpea cofactor

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99987
Spacegroup name	I 41 2 2
Unit cell lengths	57.510, 57.510, 183.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.890 - 1.750
R-factor	0.1742
Rwork	0.173
R-free	0.19690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.122
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.890	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.101	0.495
Rpim	0.037	0.191
Number of reflections	15226	796
<I/σ(I)>	11.8
Completeness [%]	94.9
Redundancy	7.3
CC(1/2)	0.995	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	298	ammonium sulfate, sodium acetate