9EBV

Structure of the Bacillus subtilis yjdF riboswitch aptamer domain in complex with lumichrome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU MICROMAX-003
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-22
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54178
Spacegroup name	P 21 21 2
Unit cell lengths	110.718, 68.927, 82.654
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.793 - 2.519
R-factor	0.2185
Rwork	0.216
R-free	0.24630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.774
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.590
High resolution limit [Å]	2.500	2.500
Rpim	0.040	0.322
Number of reflections	21630	1685
<I/σ(I)>	20.63
Completeness [%]	97.4	77.6
Redundancy	4.8	3.4
CC(1/2)		0.854

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	6% MPD, 9 mM spermine, 40 mM Na-cacodylate pH 7.0, 20 mM KCl