9EB8
Crystal Structure of Biotin Carboxylase from Ankistrodesmus in Complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS SIRIUS BEAMLINE MANACA |
| Synchrotron site | LNLS SIRIUS |
| Beamline | MANACA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-08-15 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.977140 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.777, 95.362, 95.194 |
| Unit cell angles | 90.00, 92.67, 90.00 |
Refinement procedure
| Resolution | 47.680 - 1.900 |
| R-factor | 0.1797 |
| Rwork | 0.178 |
| R-free | 0.21670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.880 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.680 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.302 | 1.359 |
| Rmeas | 0.337 | 1.530 |
| Rpim | 0.147 | 0.690 |
| Total number of observations | 620270 | 28468 |
| Number of reflections | 124358 | 6126 |
| <I/σ(I)> | 5.4 | 1.4 |
| Completeness [%] | 99.6 | |
| Redundancy | 5 | 4.6 |
| CC(1/2) | 0.913 | 0.443 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M ammonium sulfate, 0.1 M Bis-Tris, pH 6.5, 25% w/v PEG3350 |
