9EB6

Chicken YF1.7*1 presenting myristoylated peptide derived from tegument protein CIRC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	I 1 2 1
Unit cell lengths	55.289, 55.410, 137.904
Unit cell angles	90.00, 97.92, 90.00

Refinement procedure

Resolution	38.950 - 1.900
R-factor	0.1738
Rwork	0.171
R-free	0.22060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.108
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.0.32)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.950	1.968
High resolution limit [Å]	1.900	1.900
Rmerge	0.032	0.319
Rmeas	0.045	0.451
Rpim	0.032	0.319
Number of reflections	32495	3219
<I/σ(I)>	13.88	2.27
Completeness [%]	99.2	98.65
Redundancy	2	2
CC(1/2)	0.999	0.798

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	18% w/v PEG 4000, Sodium acetate