9EB5

Chicken YF1.7*1 presenting myristoylated peptide derived from tegument protein MDV023

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	I 1 2 1
Unit cell lengths	57.116, 55.429, 133.412
Unit cell angles	90.00, 101.43, 90.00

Refinement procedure

Resolution	48.130 - 2.080
R-factor	0.189
Rwork	0.186
R-free	0.24270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.937
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.0.32)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.130	2.154
High resolution limit [Å]	2.080	2.080
Rmerge	0.046	0.254
Rmeas	0.065	0.360
Rpim	0.046	0.254
Number of reflections	24812	2461
<I/σ(I)>	11.03	2.86
Completeness [%]	100.0	99.92
Redundancy	2	2
CC(1/2)	0.998	0.847

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% w/v PEG 4000, 200 mM magnesium sulfate, 10% v/v glycerol