9EB4

Chicken YF1.7*1 presenting N-palmitoylated glycine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95366
Spacegroup name	I 1 2 1
Unit cell lengths	56.669, 55.390, 133.456
Unit cell angles	90.00, 100.98, 90.00

Refinement procedure

Resolution	51.020 - 2.200
R-factor	0.1606
Rwork	0.157
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.910
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.0.32)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.020	2.279
High resolution limit [Å]	2.200	2.200
Rmerge	0.040	0.098
Rmeas	0.056	0.140
Rpim	0.040	0.098
Number of reflections	20758	2036
<I/σ(I)>	12.74
Completeness [%]	99.7	98.93
Redundancy	1.9	1.9
CC(1/2)	0.994	0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% w/v PEG 4000 200 mM magnesium sulfate 10% v/v glycerol