9EAF

Carboxyspermidine decarboxylase from Clostridium leptum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	63.570, 80.850, 140.535
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.850 - 1.410
R-factor	0.1504
Rwork	0.150
R-free	0.18920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.730
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.850	1.450
High resolution limit [Å]	1.410	1.410
Number of reflections	133970	6885
<I/σ(I)>	12.9
Completeness [%]	95.8
Redundancy	13.1
CC(1/2)	0.999	0.923

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	298	potassium phosphate dibasic, PEG 8000, glycerol