9E9Q

SARS-CoV-2 SL5 crystal structure native

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.59980
Spacegroup name	P 32 2 1
Unit cell lengths	96.717, 96.717, 114.823
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.360 - 3.330
R-factor	0.1626
Rwork	0.158
R-free	0.20430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.357
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.360	3.449
High resolution limit [Å]	3.330	3.330
Rmerge	0.069	3.428
Number of reflections	17556	1721
<I/σ(I)>	15.35	0.67
Completeness [%]	99.8
Redundancy	10.4
CC(1/2)	0.997	0.246

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	1.2-1.4 M sodium citrate, 0.1 M HEPES-NaOH, pH 6.5-7.5 reservoir mixed in ratios of 1:2, 1:1, or 2:1 with folded RNA solution containing 25 mM HEPES-KOH, pH 7.4, 150 mM KCl, 10 mM MgCl2