9E92

Acanthamoeba Polyphaga Mimivirus R699

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	110
Detector technology	PIXEL
Collection date	2023-02-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	69.153, 124.998, 72.757
Unit cell angles	90.00, 118.30, 90.00

Refinement procedure

Resolution	60.965 - 1.500
Rwork	0.166
R-free	0.18900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.800
Data reduction software	xia2 (3.8.0)
Data scaling software	Aimless (0.7.8)
Phasing software	PHENIX (1.20.1)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.500	1.520
High resolution limit [Å]	1.500	1.500
Rmerge	0.059	0.602
Number of reflections	171464	49803
<I/σ(I)>	13.3	1.8
Completeness [%]	98.4
Redundancy	6.8	6.3
CC(1/2)	0.998	0.857

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Protein/substrate/cofactor mix: 28 mg/ml R699, 10 mM UDP glucose, 10 mM MnCl2, 0.15 g/ml glucose, 0.15 g/ml galactose. Mother liquor: 0.1 M HEPES pH 7, 10% (w/v) PEG 6000. The ratio of mix and mother liquor was at 1:1.