9E7O

Pfs230 D13D14 in complex with nanobody W2810

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953647
Spacegroup name	C 1 2 1
Unit cell lengths	135.494, 62.824, 56.481
Unit cell angles	90.00, 98.92, 90.00

Refinement procedure

Resolution	39.920 - 1.930
R-factor	0.2158
Rwork	0.214
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.028
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.920	5.750	2.050
High resolution limit [Å]	1.930	4.080	1.930
Rmerge	0.048	0.019	0.642
Rmeas	0.088	0.037	1.364
Number of reflections	35422	2458	5608
<I/σ(I)>	13.83
Completeness [%]	99.9
Redundancy	7
CC(1/2)	0.999	0.999	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.4 M sodium malonate dibasic monohydrate pH 7.0