9E7D
Crystal structure of HIV-1 RRE SLII A31C mutant in complex with Fab BL3-6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 |
| Unit cell lengths | 72.874, 75.871, 83.516 |
| Unit cell angles | 63.17, 71.85, 77.75 |
Refinement procedure
| Resolution | 34.200 - 3.110 |
| R-factor | 0.2497 |
| Rwork | 0.246 |
| R-free | 0.32900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.364 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.200 | 3.160 |
| High resolution limit [Å] | 3.100 | 3.106 |
| Rmerge | 1.446 | |
| Rpim | 0.934 | |
| Number of reflections | 21022 | 3048 |
| <I/σ(I)> | 1.5 | 0.6 |
| Completeness [%] | 99.0 | 98.6 |
| Redundancy | 3.5 | 3.4 |
| CC(1/2) | 0.779 | 0.319 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 298 | 0.2 M Ammonium nitrate, 20% PEG 3,350, pH 6.2 |
