9E7A
Crystal structure of the MIR domain of the S. cerevisiae mannosyltransferase Pmt4 in Complex with Ccw5
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-16 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.078 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.599, 78.850, 118.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.750 - 1.690 |
| R-factor | 0.1913 |
| Rwork | 0.190 |
| R-free | 0.22660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.130 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MrBUMP |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.750 | 1.710 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.034 | 0.198 |
| Number of reflections | 94643 | 4755 |
| <I/σ(I)> | 11.54 | |
| Completeness [%] | 99.4 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% polyethylene glycol monomethyl ether 2000 at pH 8.0 |
