9E79
Crystal structure of the MIR domain of the S. cerevisiae mannosyltransferase Pmt4
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-16 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97931 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.091, 54.942, 118.661 |
| Unit cell angles | 90.00, 90.08, 90.00 |
Refinement procedure
| Resolution | 14.830 - 1.640 |
| R-factor | 0.2116 |
| Rwork | 0.199 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.867 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2-5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.580 | 1.670 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.044 | 0.235 |
| Number of reflections | 163344 | 2222 |
| <I/σ(I)> | 12.48 | 1.32 |
| Completeness [%] | 97.2 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.0 M LiCl, 0.1 M citric acid, and 30% PEG 6000 (w/v) at pH 8.0 |
