9E6P
Structure of a Mouse KC dimeric mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-06-03 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 44.859, 72.184, 42.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.460 - 3.200 |
| R-factor | 0.2365 |
| Rwork | 0.231 |
| R-free | 0.28010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.736 |
| Data reduction software | HKL-2000 (v722) |
| Data scaling software | HKL-2000 (v722) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.350 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmeas | 0.351 | 0.975 |
| Rpim | 0.121 | 0.682 |
| Number of reflections | 2854 | 302 |
| <I/σ(I)> | 7.8 | 1.4 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 8 | 8.1 |
| CC(1/2) | 0.931 | 0.731 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 12% PEG-500MME, 6% PEG-20000, 100 mM of Bicine-Tris (base) pH 8.5, with 100 mM of Molecular Dimension's Carboxylic acids mixture II |
