9E6O
Structure of a Mouse KC Filament
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-04-17 |
| Detector | MAR CCD 300 mm |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.092, 42.065, 165.794 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 165.790 - 3.600 |
| R-factor | 0.2866 |
| Rwork | 0.286 |
| R-free | 0.30290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.929 |
| Data reduction software | HKL-2000 (2.3.12) |
| Data scaling software | HKL-2000 (2.3.12) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 165.790 | 3.730 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.850 | |
| Rmeas | 0.113 | 1.215 |
| Rpim | 0.056 | 0.602 |
| Number of reflections | 12807 | 1526 |
| <I/σ(I)> | 13.9 | 1.23 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4 | 4.1 |
| CC(1/2) | 0.985 | 0.592 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 8% PEG-500MME, 4% PEG-20,000, 100 mM of Bicine/Tris (base) pH 8.0, with 10 mM of Sodium nitrate, sodium phosphate dibasic, Ammonium sulfate. |
